Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

I need to accelerate corrosion in steel parts that are used in a salt-water environment. Is there a technique for doing so?

I have a structure that employs d-ring swiveling tie-downs that corrode during use and become "frozen". I have sample swivel assemblies that I wish to corrode to see if I can free them using one...

01 March 2021 3,739 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

Does anyone ever solved this following Matlab error : "Error using imaqdevice/start (line 95) pointgrey: Failed to stop the camera acquisition." ?

I use Matlab 2014b to capture some images with a PointGrey camera connected with USB3 port. I initialize the camera in a function which basically execute these followed lines : vid =...

22 February 2021 6,386 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View

What are the main conditions suited for synthesizing a PVDF thin film by solvent casting method?

I have been trying to synthesize PVDF thin film by solvent casting method. I started by using 2:3 DMSO: acetone as solvents. The solution was stirred for 3hrs at 60 deg and was dried at 90 degrees...

09 February 2021 8,850 3 View

Wave velocity and rock density paradox?

Practically it is known that seismic velocity increases as rock density increases, why then does the wave velocity equation involving elastic moduli and density indicate that velocity is inversely...

09 February 2021 1,843 3 View

Frequency calculation with TDDFT???

Hi, I have been trying to calculate the frequency vibrations of the excited state S1 of an organic molecule (24 atoms). The geometry optimisation of the excited state converged smoothly howerver...

22 January 2021 1,463 3 View

No title

Greetings to all. I have some questions about setting up DFT calculations in Gaussian software (I am a beginner in these matters), and trying to calculate the energy of the first excited triplet...

06 December 2020 3,504 4 View

Where and how do I specify frequency in the route section of frequency-dependent calculation (Gaussian using Linux)?

Hello everyone. I would like to compute the dynamic hyperpolarizability value of a molecule using Gaussian on Linux OS. I have read that the essential keywords to include for such request are the...

07 November 2020 5,620 4 View

What is the algorithm used by Gabedit for reading an Excited States Gaussian Output File and plotting the UV-Vis spectra of molecule?

I would like to know if Gabedit uses the GaussView algorithm for plotting UV-Vis spectra from excited states calculations output files that is shown in https://gaussian.com/uvvisplot/, or if...

23 September 2020 9,459 3 View

How do I calculate theoretical fluorescence spectra (TDDFT) in Gaussian 09W in selected solvent?

Good day everyone. I am new to computational chemistry and would like to integrate some part of it in my fluorescence study. I would like to investigate the theoretical fluorescence spectra using...

18 September 2020 6,742 3 View

Tried to do Freq calculation for a organic molecule in ORCA but the calculation is still running. What should be the steps to make it faster?

I am running Freq calculation combined with optimization of a molecule having 14 carbon atom but the calculation is still now running since 3 days. I also have done only optimization of the same...

22 August 2020 2,004 3 View

Problem with the optimization of second excited state?

For the optimization the second triplet excited state of small molecule based on the BODIPY, I tried to calculate it with the keyword 'root=2', but the result was the first excited state not the...

18 August 2020 432 1 View

Solve the problem ORCA error running (CoPc(OH)16)?

Hello, I did a running iron and cobalt phthalocyanine compound plus functional groups -OH (FePc(OH)16) & (CoPc(OH)16) with ORCA using Gabedit. when running CoPc with use: # CoPcOH16 ! B3LYP...

11 August 2020 9,990 2 View

Why in TDDFT, some of the output for the first state has a negative wavelength and oscillator strength ?

I'm doing TDDFT calculation. I have calculated excited state calculation for n=20 states. The first state (lowest in energy) showing negative wavelength and oscillator strength. How we can explain...

11 August 2020 6,788 4 View

How to predict accurate Fluorescence/Vertical Emissions employing TD- DFT? Excited State Tuning Approach?

How can we predict accurate Fluorescence wavelengths employing DFT? Which is the most accurate DFT methodology currently available which can predict accurate Fluorescence wavelengths (Vertical...

05 August 2020 4,345 1 View