Is there a validated questionnaire assessing participant enjoyment and adherence of an exercise intervention?

More Ethan L Ostrom's questions See All

What would cause a cell that was plated in two different densities to become the same confluence 4 days after?

I seeded 5e6 and 3e6 SW756 cells in two 15cm dishes with equal amounts of media on Thursday, anticipating one to be confluent Monday/Tuesday, and the other to be confluent sometime later, maybe...

28 January 2024 8,844 1 View

How do I layer Cells on one another?

Hi, I have extensive 2D and 3D spheroid cell culture experience and I am considering a multi-cellular culture whereby I layer cell types on top of each other, for example stromal fibroblasts...

23 October 2023 2,131 2 View

Why does HEK293 FT morphology look strange?

I have worked with HEK293 FT cells for many years. Recently, they have mysteriously started to look abnormal (see attached). After being passaged and grown in complete DMEM media (10% FBS,...

16 October 2023 3,197 6 View

Practical ways of applying Piezoelectricity in renewable enegy?

Additional application of Piezoelectricity in renewable energy would be a huge step towards the growing industry. Is there any possibility or possible combinations of existing renewable energy...

19 August 2023 1,220 1 View

Is there an oxidative stress dye labeling method, where the dye sensor is added before the oxidant?

Many CellROX DNA dye cell labeling methods need test ROS compound preincubation. Then when you add the CellROX dye, the dye oxidizes with the ROS and binds to the cell's DNA to fluoresce. Is...

05 April 2023 6,373 2 View

Ceiling Temperature Database?

I'm interested in identifying a polymer with ceiling temperature between 0-35 Celsius. Does anyone know of some database with information on the ceiling temperature of various polymers? Ideally...

21 March 2023 8,634 3 View

Why does the absorbance at 340nm decrease when it should increase for my enzyme activity assay?

Hi, I’m measuring the production of NADH so the A340 should be increasing but instead its decreasing. Its even more strange because I blank and autozero before I add the NAD. My enzyme is...

06 February 2023 8,921 3 View

Which does E. coli prefer more as a carbon source, sorbitol or glycerol?

The reason why I ask is because I want to add sorbitol to be terrific broth with glycerol but I'm worried it will preferentially metabolize the sorbitol. I want to add sorbitol because I've read...

04 January 2023 6,973 3 View

What's causing such a low quality with my sanger?

I've started doing sanger sequencing recently and only rarely get good results. Every run I do barely has >100 Q20 and always has weak signal and excessive background noise. I've attached an...

10 November 2022 2,359 2 View

Optimizing PCR conditions around varying primer Tm?

Hello, I've been tasked with validating a 2kb region of DNA. A colleague designed primers with the F melting at 53.8 and the reverse at 59.7. I believe they used 54C which had multiple bands show...

16 October 2022 5,400 6 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Why wait for a doctor's visit when you can become the guardian of your child's health today?

Join our exclusive WhatsApp group and access the latest research-based videos on children's health! Discover the hidden toxins and other risks that may harm your kids, and learn the best practices...

04 August 2024 1,264 0 View

Ready to take control of your child's health and well-being?

Join our exclusive WhatsApp group and gain access to the latest research-based videos on children's health! Uncover the hidden toxins and other risks that may threaten your kids, and equip...

04 August 2024 5,895 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View