Hello, there are so many ways to analysis the expression of gene and mRNA, but I wonder if we have a general unit to specific the expression of a gene (and this unit can use for all of methods)? I mean, if gene expression can have their own unit, based on the unit we can compare the expression of genes together, and the expression of that gene in this tissue to another tissues, and we can say it is house-keeping gene or tissue-enriched? Thank you.
You could use the copy number in absolute quantification for this purpose.
Also it is possible to use calibrator in relative quantitation experiment. As a result you can receive percents (%).
I am thinking of the unit such as " mol per 1 unit of volume". We can use the "fold change" of course. But I just wonder if we can use the unit. Because, there can be a lot of variation (obscuring variation) when we do experiment, I just wonder if fold change can be use to compare between tissue to tissue with regards to only one gene. In the case that I want to draw a chart of "the gene expression of only one gene in many types of different tissue" or "the gene expression of only one gene in many different status of one cell".
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