Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View

What is the best way to differentiate L. rhamnosus and L. casei in plate co-cultivation?

I'm having troubles in the differentiation and quantitation of each bacteria population during co-cultivation. The morphologic characteristics are very similar and it's really difficult to count...

13 January 2021 1,550 1 View

Have you ever included two last authors in the co-authorship of a publication?

Hello, As you must know, it is now sometimes possible to have several co-first authors in the co-authorship of a scientific publication, when two persons led the paper and had equal contribution...

21 September 2020 2,995 5 View

Which is the viscosity recommended for using Methyl cellulose to perform oral gavage (mice )?

Hello, we would like to perform oral gavage in mice. The vehicle recommended for our drug consist on 0.5% methylcellulose and 5% Dimethylacetamide (0.1 ml/ 10g mouse). Could somebody help us to...

20 August 2020 8,720 2 View

How does internal resistance affects the self discharge in a super capacitor?

If ESR doubles for instance, what should we expect for self discharge?

30 May 2019 3,190 0 View

Article - Research to evaluate the compaction of soils under > 2 years test ?

13 August 2018 9,973 4 View

Wich are the best combination of Restriction Enzimes for double digestion?

I want to do a RFLP assay for DNA of bacteriophages. in this case I want to performance double digestion because not all restriction enzimes cut the specific sequence of strands of DNA. thanks...

06 July 2018 4,134 1 View

Why is RFLP technology so popular for phage studies?

In many papers I see that is so popular the use of RFLP for phages studies? why not use others techniques (PCR,).

04 May 2018 8,407 0 View

Exosome Quantification

24 May 2017 3,750 3 View

Somebody had use Millipore ECM590 | Blood Vessel Staining Kit?

Im trying to use it with cow tissue, but I don't know if Im doing the protocol right.

24 August 2016 3,467 1 View

How do we calculate curvature value in ArcGIS?

Hello everyone, I am trying to prepare curvature map in ArcGIS 10.4, using spatial analyst tool. I am using 10 m CartoDem (projected in UTM 44N). I am getting very different value, curvature...

12 January 2021 504 4 View

What is the best GIS tool to calculate connectivity score for a point along aquatic network?

Hello, I am looking for a GIS tool (ArcGIS, QGIS, R, etc.) that will calculate a measure of habitat connectivity for nodes along an aquatic (estuarine) network? My dataset is comprised of a...

12 January 2021 6,107 2 View

How can I increase the latitude and longitude scaling to clearly show two close points in QGIS?

I have three points to show where one point is very far from the other two very closely placed points. Zooming the map doesn't work. So I want to increase the scale of the latitude axis only to...

07 January 2021 8,619 2 View

Can any one answer me how to prepare LULC map using QGIS?

Working on watershed delineation

01 January 2021 2,604 5 View

Is there a way to automatically gather surface area from a 3D model based on color/texture?

I have been constructing 3D models of large corals and I was wondering if there is a software that I can use to gather the area of live tissue vs dead/disease? I am aware there is a way by tracing...

17 December 2020 6,991 1 View

Which among the standard colour palettes available in QGIS are colour-blind friendly?

I'm producing a map with several habitat typologies (14) and it's a struggle to find a colour combination that would be fully colour-blind friendly. QGIS offers the possibility to check your image...

29 November 2020 1,397 3 View

Converting csv files with data for mapping rainfall on QGIS?

Hi, I am hoping to produce rainfall and temperature maps for a current project in QGIS. I have csv data downloaded which have been imported into QGIS. My aim is to be able to produce rainfall...

10 October 2020 4,930 5 View

Suggestions how to get Shapefile of India with all 700+ Indian districts?

I need shapefile for all 700+ districts of India. From where I can find the India shapefile with all districts. Also, suggest how to get the latest India shapefile with all state and union...

07 August 2020 3,449 5 View

How to calculate the distance between points in Qgis?

I have a shape file containing almost 3000 points and a delimited text layer with almost 500 points. What I want to do is to calculate the distance between each 500 points and the nearest point to...

06 July 2020 3,709 10 View

How to convert GeoTiff to a format compatible with FRAGSTATS?

Dear all, I need to process several classified GIS maps in FRAGSTATS. All files are in GeoTiff format. I do not have ArcGIS. I also assume that when you export files to ASCII format in QGIS often...

01 July 2020 2,433 5 View