Is there a substitute for Hygromycin B to be used for selection?

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Ajonok Hano

You can order alternate antibiotics, click on this website http://www.thermofisher.com/cn/zh/home.html

Christian Q. Scheckhuber

If you want to do a selection of transformants based on the hygromycin resistance gene, Hygromycin B is probably the only option you have. Other antibiotics like phleomycin would require to exchange the hygromycin resistance cassette on your plasmids.

Best regards,

Christian

Keun Kim

I did a yeast research long time ago and not doig these days. You can try Google-search for selection marker for industrial yeast . Also there is a company selling plamid for that selection. I do not remeber the company name.

Prachi Nawkarkar

thank you for your opinions Christian , Keun and Aquleem .