How can I prevent gene expression before collecting microorganisms by centrifugation?

Hy everybody, I am looking for a way to stop gene expression during harvesting of microalgae to ensure reliable RNAseq in response to temperature. I am affraid working on ice and performing...

25 November 2020 7,282 3 View

How to increase the efficiency of overexpression using lentiviral TET-ON system in primary kidney cancer cells?

I am using a lentiviral TET-ON system for conditional overexpression of my gene of interest in primary kidney cancer cells. I use viral load from transfection of HEK293T cells after 48 and 72...

16 November 2020 2,496 1 View

Has any endophytic microbe been isolated from the bark of coffee plant?

Any bacteria or Fungi from the barks of coffee

10 September 2018 2,993 4 View

Very minimal researches published on online businesses even though online businesses is gaining moment world wide due to ease access of the internet?

Very minimal researches published on online businesses even though online businesses are gaining momentum world wide due to ease access of the internet?

22 May 2018 4,022 3 View

What is the maximum solubility of RNA ?

I am looking for publications (or patents) stating the solubility of RNA (either total RNA, tRNA, whatever the species) in water or in saline solutions. Thanks for helping me !

19 September 2017 9,880 5 View

Can someone provide a protocol for building yeast strains using Tet-Off expression system?

I am trying to express my gene of interest using a Tetracycline inducible vector. Although I need my gene of interest of be off and only be turned on for a short period of time when needed, I only...

07 July 2017 3,994 19 View

How to troubleshoot qPCR primer optimizations?

I am having some issues with primer optimization for qPCR. Typically, I first test various concentrations of primers to figure out which concentrations gives the best melting curves and the lowest...

17 January 2017 9,923 2 View

Xylan from beechwood discontinued by Sigma-Aldrich

28 August 2016 3,993 7 View

Hi! can anyone help me identify this plant from a lake in the Philippines which is constantly being affected by fish kill?

07 January 2016 2,793 3 View

Do plasmids need to be CIP treated for plasmid religation assay?

I am trying an experiment in which I measure the NHEJ efficiency of a mutant yeast strain and compare to wild-type to analyze whether the mutation affects the NHEJ process. I take a low copy...

16 December 2015 2,586 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View