Hello,
I am wanting to express mouse skeletal muscle contractile performance to lean tissue mass, and I had been advised in a piece of peer review for a paper that it is possible to perform such an analyses by placing skeletal muscles into chloroform methanol for several days, drying, and expressing contractile performance to the remaining lean tissue mass. I am struggling to find anything in the literature to have used this approach for extracting lipids from mouse skeletal muscles.
Is there a published protocol regarding this analyses, or does anyone have a step-by-step guide they could direct me to? Or is it really as simple as the above procedure?
Lance Bollinger
Here is a quick methods paper describing lipid extraction from muscle tissue (human).
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