I have seen hybridization assays (detecting hybridization events) using both EIS (electrochemical impedance spectroscopy) and DPV (differential pulse voltammetry). I have seen less of EIS being published in literature.
Differential Pulse Voltammetry and EIS are both electrochemical techniques, but they have different goals. DPV is an "analytical" technique that is usually used to measure the concentration of some specific electroactive species. It is very sensitive and, along with Square Wave Voltammetry, the most common pulse technique.
EIS, on the other hand, is used to 'characterize" any electrochemical system. EIS is rarely, if ever, used to determine the concentration of an analyte. Instead, it measures the impedance of an electrochemical system over a wide range of frequencies. EIS provides insight on the kinetics of multiple electrochemical processes as well as information on the capacitance of the system.
In order to use DPV, you should have substances that has reduction or oxidation signal (guanin base or Meldola Blue ....etc)....For EIS, you can uderstand hybridization event by evaluating resistance differences. DPV needs less time and more sensitive..
EIS has some disadvantages such as false positive signals which produced by adsorption, it must be carefully considered especially in DNA. I have experence about it was really hard to examine the true signals.
Differential Pulse Voltammetry and EIS are both electrochemical techniques, but they have different goals. DPV is an "analytical" technique that is usually used to measure the concentration of some specific electroactive species. It is very sensitive and, along with Square Wave Voltammetry, the most common pulse technique.
EIS, on the other hand, is used to 'characterize" any electrochemical system. EIS is rarely, if ever, used to determine the concentration of an analyte. Instead, it measures the impedance of an electrochemical system over a wide range of frequencies. EIS provides insight on the kinetics of multiple electrochemical processes as well as information on the capacitance of the system.
- Badges
- Science topic
- Similar topics
- Chemistry
- Electrochemistry