How could I determine the starting methyltransferase enzyme and methyl donor to methylate a synthetic peptide?

Hello, I have a synthetic peptide with arginine residue I aim to methylate using PRMT1 + SAM (methyl donor). I then will use my dimethylated arginine peptide product in ELISAs and Western blots....

12 November 2020 5,568 7 View

ELISA-Why does my diluted high absorbance sample have such lower concentration compared to less diluted sample when using standard curve?

Hello! Our lab has developed a standard curve with known concentration that we use to determine our ELISA concentrations. We then multiply by the dilution factor for our final concentration....

25 October 2020 8,601 6 View

How to handle the analysis of yield stability or yield risk based on simulated crop yield data?

Dear everyone, do you have any experience on the analysis of yield stability or yield risk based on simulated crop yield data? I would like to use a modelling approach and simulate grain yields...

23 February 2020 1,584 6 View

What is the best way to calculate the yield of an entire crop rotation including different kind of crops ?

What is the best way to calculate the yield of an entire crop rotation including different kind of crops and different yield levels ? For example the following cropping sequences: 1. sugar beet...

03 December 2019 9,527 11 View

Protocol for whole heart tissue lysis for Westerns?

Hello, I am troubleshooting a western protocol using whole heart homogenates but I seem to be getting consistent smearing at the lower band range of my gel which is also where I want to see my...

01 October 2019 8,682 4 View

What is the minimum sample size for factor analysis?

I will be doing ryff's psychological well-being scale for psychometric properties. What would be the minimum sample size for factor analysis using spss? I read Slovin's formula is not reliable?

26 April 2017 601 7 View

Could alternate form reliability be administered simultaneously?

I will be doing ryff's psychological well being scale's psychometric properties. Could I administer alternate form reliability simultaneously? (42-item and 18-item)

25 April 2017 8,184 3 View

IS Confrimatory Factor Analysis CFA and WLS enough for psychometric evaluation?

Im going to do a psychometric evaluation on ryff's scale using nurses as my sample. Would CFA and WLS suffice? Thank you!

13 April 2017 4,938 3 View

Can Zung's Depression Scale be administered by a student?

I am currently doing my psychometric evaluation on ryff's scale and would like to use Zung's scale for my divergent validity. I am an undergrad student. Can I administer it?

09 April 2017 510 5 View

What would be a good discriminant validity for Ryff's PWB?

04 April 2017 5,633 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

How to use bag of words for action prediction?

Hi everyone, I'm working on an action prediction (i.e., early recognition) using Bag-of-Words. I know how to use BOW, but regarding action prediction, there is a subtle problem. In the...

19 February 2021 1,442 4 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

What are the available keys and descriptions for the identification of lice and fleas in domesticated animals?

I am an undergraduate student at the University of Peradeniya Sri Lanka, currently working on ecto-parasites in domesticated animals. I found difficulties in the identification due to a lack of a...

15 February 2021 277 1 View

Does learning mathematics exclusively improve cognitive abilities?

Teaching Mathematics at school to all students is commonly justified by the opinion that it improves their problem-solving skills and "makes them smarter" (whichever measure is implied by this). I...

15 February 2021 7,391 7 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View