How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

How do you use factor analysis on categorical variables?

Hi, I'm trying to identify how similar different individuals are in their body traits. Normally, I would use a PCA or DFA, depending on my question. However, I have only categorical variables...

20 July 2020 4,171 1 View

What is the difference between chemical and pythochemical analysis? Why must both the aqueous and alcoholic extract be made?

I'm currently studying on the pythochemical analysis of Lepisanthes alata for its antimicrobial properties. Please help me out with relevant facts and journals. Thank you in advance :)

17 March 2019 5,590 4 View

Does anyone do in vitro inhibition studies against T. cruzi trypanothione reductase, cruzain or another targets?

Hi! I'm a PhD student from the Institute of Tropical Health of the University of Navarra that have been synthesized different chemical compounds. I would like tested them against T. cruzi...

28 April 2018 8,489 2 View

Is it possible to perform bench work without a hood?

I'm planning to do an experiment in my lab (cell culturing) and the problem is that my lab does not a hood for for proper sterilization. Is there any possible was to perform lab work without using...

08 December 2016 5,797 2 View

Is it possible to use multiple stains on same cell line and analyze them by flowcytometry?

About flowcytometry.

22 August 2016 7,357 5 View

Is there any advisable online web resource for reviewing cell cytometry?

I am constantly reviewing lots of markers, stains, techniques wasting a lot of time searching over multiple pages. Does anybody recommend online resources that compile this kind of things. Best...

03 April 2016 4,424 1 View

What are the parameters of difference between atmospheric temperature and land surface temperature?

I wonder if there is a correlation coefficient or parameter difference between the land surface and atmospheric temperature. The reason for this is to obtain information from the atmospheric...

03 April 2014 7,865 3 View

Does anyone know how to solve problems related to non-specific binding in sandwich ELISA?

Does anyone know how to solve problems related to non-specific binding in sandwich ELISA for the quantification of pertussis toxin and pertactin from Bordetella pertussis?.

26 August 2013 4,475 3 View

How does one use the product MOD43A3 (Albedo) in environmental studies?

It's the first time I have used MOD43A3, I would like to know how to use the images referred to as black-sky albedo and white-sky albedo. Which is used for environmental studies? I'm looking to...

15 May 2013 1,904 1 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT?

Im looking for any papers, reports,articles, links etc on information on How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT.

24 February 2021 2,750 4 View

How to perform Kaplan-meier survival analysis in SPSS?

Hi everyone, This is my first time of attempting to run survival analysis. I have a data on patients with End-stage Kidney Diseases (ESKD). I would want to run survival probability by treatment...

16 February 2021 2,647 3 View

What is the optimal method to prepare a controlled porosity osmotic pump (CPOP) tablet for scanning electron microscopy?

In order to assess the shape and size of pores formed by dissolving the pore former in the semipermeable membrane structure, SEM images are needed. Cellulose acetate was used in the coating...

15 February 2021 6,135 3 View

Do the introduced salts (NaOH/ HCl) influence the spectroscopic shifts of the fluorescence excitation-emission matrix?

During the resin (XAD-8) fractionation of wastewater salts (NaOH or HaCl) are used for acid or base elutions/pH adjustments. Since the amount of introduced salt is minimal, so is it required to...

08 February 2021 9,489 2 View

Is carboxymethyl cellulose a better electrolyte host polymer than methyl cellulose and cellulose acetate?

I want to prepare a cellulose-based biopolymer solid electrolyte by solution casting technique. However, choosing the right cellulose derivative has been my major challenge. Among the many factors...

30 January 2021 5,355 3 View

Are there any technical and safety tips or details for hot salt baths?

29 January 2021 6,036 2 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View