Need restriction map to plan construct
Hi, Recently I was running VASP to calculate the spontaneous polarization of my alloy structure. I followed the instructions in this PDF wrote by Dr. Nabin (Nabaraj) Pokhrel, below is my INCAR...
07 August 2024 486 5 View
Who are some key author to refer to?
18 May 2024 7,998 5 View
Dear Colleagues, We are conducting a meta-analysis to synthesize comparative studies investigating the relationship between proxies of sexual selection and species richness or speciation rate in...
07 April 2024 5,425 0 View
I added 4mass% also get the results of beads clumped together. Is it because the ethanol does not mix well with PET/HFIP?
29 March 2024 2,164 0 View
I have prepared two same materials with diffferent method and performed electrochemical testing. The electrode A which has higher specific capacitance gives higher (8 times) solution resistance...
25 March 2024 5,833 0 View
In the JCQ, the items are scored on a five-point Likert scale from strongly disagree (score of 1) to strongly agree (score of 4). I read some studies they showed scores on the job demands scale...
23 March 2024 8,362 2 View
Hi, I am doing a pulldown in THP-1 wt lysate followed by MS-based proteomics. However I get a very low protein amount out of the cells (1 mg per 12 million cells). I use the following lysis...
14 February 2024 9,030 1 View
Hi there. I have to use the pET28a-SUMO vector to get my target protein. However, I can‘t separate it from SUMO tag. My protein was about 15 Kda big and has a about 5 value of pI. Due to the...
09 January 2024 2,970 4 View
Is the cif file for MXene(Ti3C2Tx) available? If yes, can anyone provide me with one for performing rietveld refinement? I have search from COD and AMCSD website and can't seem to find one.
13 November 2023 5,000 2 View
Why is it that when I search for literature on 3D printing bone scaffolds using materials like PLA, I seem to find more studies using FDM rather than SLA? And I have another related question: I...
11 September 2023 5,641 3 View
how can I read Waters, R. S. 1958. Morphological mapping. Geography 43 :10-17 from internet? note: not in google or resaearch gate
26 July 2024 7,813 3 View
I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.
25 July 2024 6,505 2 View
Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...
20 July 2024 9,705 0 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
I am currently using a map downloaded from https://gadm.org/maps/DEU/bayern.html and I would like to assign the value of a variable to each cell with R. I was also trying to get this information...
15 July 2024 8,844 1 View
I would like to do peptide map of a protein which is attached to PEG via lysines. Now the best approach would be to de-PEGylate it but then do the peptide map. But there is little literature on...
14 July 2024 5,634 2 View
Dear all, I have a difference in the weight ratios between EDS mapping. What should I rely on? Is there a way to solve this problem?
12 July 2024 305 1 View
I was trying to model land cover prediction using CA-Markov in Terrset 2020, and one of the input is suitability maps that incorporated data such as elevation and slope, is anyone know how to...
30 June 2024 509 2 View
Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...
29 June 2024 9,688 1 View
