No title

02 March 2021 3,060 3 View

Does anyone know of tested survey questions or value statements to evaluate consumer ethics for food and agriculture?

I'm looking for tested survey questions or value statement that address topics of food and agricultural ethics and consumer values for food purchasing... is anyone aware of an existing study that...

01 July 2020 301 2 View

An idea about the range of oxidation potential of alkyl halides (especially iodide) ?

I am actually wondering what is the range of oxidation potential of alkyl halides, especially species bearing an Iodine atom. I didn't find information in my book (organic electrochemistry by Ole...

25 May 2020 1,297 4 View

What is the best DNA extraction protocol to use for extremely oligotrophic soils?

We are trying to extract DNA from soil samples, these samples are collected from the Namib Desert, the site is about 20 Kilometers from the Atlantic Ocean. The soil has a high salt concentration...

26 October 2019 9,423 3 View

Can anyone suggest a non-parametric version of a within-between repeated measures ANOVA?

06 August 2017 1,937 3 View

Fslchfiletype for SPM usage: orientation?

10 August 2016 3,004 2 View

How come my cresol purple solution (cf SOP 6b, Dickson) has no absorbance peak at 578 nm?

19 July 2016 9,230 6 View

Why does Tris has low-conductivity?

25 January 2016 2,807 1 View

Is there a workflow for analyzing microRNA data in Galaxy?

01 December 2014 1,079 6 View

Best method for bacterial DNA extraction from intestinal biopsies (small sample size)?

I have recently used the DNEasy tissue kit to extract DNA from intestinal biopsies with the goal of amplifying the 16SrRNA gene for bacteria for sequencing. I know there are many kits on the...

30 August 2012 7,436 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View