I am trying to quantify (or even be able to detect) very small concentrations of phenolic acids in protein-phenolic acid complexes. The expected concentration is really tiny 1-3 phenolic acid molecule/ protein molecule. I was thinking that a dye specific for phenolic acids would be a good way to identify phenolics acids in such situations. Quantification is optional even some qualitative results (presence vs absence of phenolic acids) will do.
Any ideas?
Mickaël Laguerre
There is a dye called Neu's reagent that consists in 2,2-diphenylborinate aminoethyl colouring phenolic acids in a specific color. This is used for TLC identification or even microscopic evaluation. I remember that it colours flavonoids differently than phenolic acids.
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