I want to study human serum properties in the absence of albumin. In order to do that i need to use a protocol that will deplete most(if not all) of albumin from my serum samples but in the same time, leave rest of serum "intact" in order to be used for further experiments.
I tried to use a protocol with NaCl and Et-OH (95%) precipitation but it doesn't seem to work for me.
Anyone can help me? :)
Antonis: Personally, I'd not do any depletion of any proteins from the serum samples, as many of them associate with others, especially for albumin. You may end with depleting a bunch of trace amount of proteins if you are looking for them.
Thanks for your interest and for answering.
The problem is that i need to study an effect of serum without albumin, in order to examine the role of albumin for the effect. That is why i really need to deplete it.
I do know that albumin as a protein interacts with many proteins and there is the "problem" of eliminating some of them but it is a "risk" i don't mind taking :)
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