I'm tying to design an experiment wherein a starter culture of cells is treated/challenged to trigger the release of cytokines into their media. This media would then be collected and added to a pool of CD4+ Th cells to simulate their receiving the cytokine signal from the original cells, and then tracked to look at changes in their differentiation into Th1 vs. Th2 state. Does anyone know if a cell line/model system similar to this exists? Can you point me in that direction if you do?
Hi,
This experiment is pointless and a waste of time and reagents to titre concentration of different cytokines in the cell line culture.
I suggest you to read the role of each different cytokines, if there is any, on naive CD4+ T cell differentiation in the absence of signal-1 (from the TCRxCD3) and signal-2 (from the co-stimulation).
If you anyway want to do this experiment, you can use primary human naive CD4+ T cells to obtain cytokines instead of CD4+ T cell lines.
All the best!
- Badges
- Science topic
- Similar topics
- Biological Science
- Cell Biology
- Culture Cells