Is their any standardized protocol Construction of Corynebacterium glutamicum vectors for producing glutamic acid?

Chintamaneni Shanmukh Chowdary @Chintamaneni-Shanmukh-Chowdary

14 August 2020 1 2K Report

Corynebacterium glutamicum is an important industrial strain used for the production of amino acids and vitamins.
So I want to know is there any standardized protocol for the Construction of Corynebacterium glutamicum vectors for producing glutamic acid.

Basically you need to carry out the following protocols: (1) Making gene construct involving required promoter (inducible/constitutive/heterologous/organism specific); (2) Inserting the gene construct to commercially available vectors to make recombinant vectors; (3) To Transform the C. glutamicum with the recombinant vector. Standardize/optimize the fermentation/growth conditions for the transformed bacteria.

For the exact protocol, you can refer manual on "Molecular Cloning". Written by Maniatis OR the one by Sambrook

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