I use the ITS sequencing method to speciation some Fusarium spp, but it give me not good result, there are 2-3 species of Fusarium very close among them, the ITS sequencing do not make difference between them.
Our group have done similar work on rubber fungal pathogen - Corynespora casiicola. We isolate the ITS region from approximately 35 fungal strains and sequence them.
Results of multiple alignment of approximately 550-600 bp ITS fragment from 35 isolates indicated sequence identity of more than 95%. We did not see much differences among the ITS sequences.
It is possible that ITS sequence may not be suitable for differentiating closely related fungal species...
We have similar results with some isolated yeast strains, however, I think SNPs (single nucleotide polymorphism) in the ITS region could be a good indicators for speciation if we use it beside other parameters (e.g Morphology, physiological,..)
It is well established that the ITS does not work for species differentiation for many Fusarium. The most widely accepted genetic region if only a single sequence is used in Fusarium is the translation elongation factor alpha. Check out Fusarium-ID (http://isolate.fusariumdb.org/index.php) for a good curated database and some other genetic regions of use.
Hello Oadi
I want to support Linda. Databases are full of information about phylogeny of Fusarium species... You just need to read it.
Additionally, may you follow this discussion about the utility of barcoding spieces based on the ITS region.
https://www.researchgate.net/post/Why_cant_traditional_taxonomy_be_replaced_by_DNA_barcoding?_sg=vjSlPBg3iu5DsRNhuzHMDUVWx4AscwZ6CzzaKo6p64r914fJRhezW05eNDEVLE1T&_tpcectx=topic_detail&_trid=53f5cea2d3df3e15338b460b_2
Sincerely
Carolina
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