I've been trying to look at the pattern of both full-length and cleaved il-1beta forms. Is it possible that all individuals (healthy) have the same ratio of full-length to cleaved forms or does the ratio differ from individual to individual? Could explain to me please, because I'm quite new in this area of research. Thank you!
Hi Nur,
pro-Il-1beta can be generated in response to LPS. A second intracellular pathway is then required to cleave pro-IL1beta into it's mature and active form. Such stimulation can be ATP binding to P2X7 receptors in immune cells by example. Overall, the ratio will vary depending on the inflammatory state of the patients you are working with.
Thank you Malika for your answer :)
I have another question.. So if it depends on the inflammatory state of the patients, theoretically those who we marked as "healthy participants" would have the same ratio then?
Ratio is different, depending on a variety of factors. In general, this is a function of an inflammasome activation. However, monocytes from healthy individuals, isolated and stimulated in a similar way, should show similar ratio of pro and mature il-1beta
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