Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

13 December 2020 9,167 9 View

How is deep learning applied in analysis of open-ended survey questions?

Hello, Can someone give me an idea on how deep learning applied in the analysis of open-ended survey questions? My intuition is that applying deep learning models for analyzing the vast...

07 December 2020 4,479 3 View

Doing a PhD research in Natural Language Processing from a statistics department...?

Hello, My situation is a little strange because I am a Statistics PhD student but I ended up doing research in Natural Language Processing (NLP)/ Deep Learning due to my supervisor's...

26 November 2020 7,293 3 View

Does good inferential comprehension first require solid literal comprehension?

Hello, This can be a silly question to ask, but my main field is not in reading comprehension. While I try my best to phrase my question, it can sound a bit awkward. Thank you for your...

11 November 2020 811 3 View

How are the 3 levels of reading comprehension reflected in grade-level English Language Arts curriculum?

Hello, How are the 3 levels of reading comprehension (literal, inferential, and critical) reflected in grade-level English Language Arts (ELA) curriculum? In particular, normally, is literal...

01 November 2020 1,240 4 View

What could be the problem in these pcr results?

All the pictures I have uploaded are gel image of genotyping. What I don't understand is, most of the time, I get very very faint second band, and I assumed it's probably a problem with primer...

28 October 2020 9,410 9 View

Is the Syntax-first model in sentence processing widely accepted, or is it facing opponents?

Hello, Is the syntax-first model of sentence processing widely accepted, or is it facing more opponents as time goes by? I know that a study in frontal-temporal network of human brain has...

23 October 2020 1,084 9 View

Is there a reading comprehension theory that suggests simple reading questions are best solved via simple text analysis, and vice versa?

My main field is not Linguistics/reading comprehension so what I am asking might sound a bit silly or not make a lot of sense. My question could certainly be better-phrased. Is there a theory in...

23 October 2020 8,310 2 View

PCR recipe with 2 reverse primers?

Should my forward primer volume in the mastermix should be same with other two reverse primers? or do I need to double the volume of forward primer so that the sum of volume of reverse primer 1...

12 October 2020 9,464 8 View

Which is a better criterion for evaluating the performance of a neural network in multi-class classification task (crossentropy loss vs accuracy)?

In terms of evaluating how well a neural network performs in a classification task with the number of classes greater than 2 (for example, classifying an observation into one of the 4 classes),...

11 October 2020 5,152 6 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View