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hello, I have several parameters (chlorophyll, primary production) that I measures at two different marine stations during 2 years (it 's a time series). I need to do a statistical test to look at...
20 June 2023 9,357 1 View
I am doing oxygen measurements using the Winkler method in marine waters under the influence of riverine inputs. Salinity never goes down 30. Recently, I am experiencing some difficulties to...
20 January 2022 1,571 1 View
Hi, Can anybody help me troubleshoot Calcium tracing? I am new at this and not sure where to begin. My protocol involves incubating cells in Fura+HBSS+calcium at 1uM in 37C for 25 min and wash...
21 June 2018 8,345 7 View
I'm looking for a human cell tracking method in mice.I can perfom a nuclear ribonucleoprotein labeling but, I would like a other tracking methodAnd also, I can't transform the human cells ! Could...
09 February 2017 2,648 3 View
Hi, I would like to understand better what the difference is between using KCl or CaCl2 or eventually H2O to extract NH4+ and NO3- from soils. Why is KCl-extraction the standard methode?Does one...
22 October 2016 3,160 2 View
Hello everyone, I am starting to work on extracellular vesicles (EVs) so recently I did an exosome isolation from cell culture media of neuroglioma cells by using ultracentrifugation protocol. I...
10 August 2016 7,752 1 View
We are looking to estimate the PP using VGPM in coastal waters such as in the Mediterranean Sea. The estimates of Zeu and Chltot are they good when the water column is only 50 m depth? Thank you!
04 July 2016 2,799 0 View
Hello, I need to send some DNA extracts to another lab, but i'm not sure about the best way to preserve DNA (it might take 2-3 days for the sample to reach my collaborators, and i afraid DNA will...
25 December 2012 8,657 19 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...
13 July 2024 2,255 3 View
