In my work, i am working on a serological proteomic analysis (SERPA) project, seeking for the antigens capable of eliciting immune responses. So just like any other typical gel-based proteomic experiment the extracted protein samples are separated through a 2D gel-electrophoresis. Then the resolved protein spots are electro-transferred (my transfer buffer is Towbin) to the immobilon-PSQ membrane, which is compatible with a mass spectroscopy experiment according to the manufacture description. Transfered proteome then will be blocked and incubated in primary and secondary antibody.
For identification of proteins by mass spectrometry, I would like to excise reactive proteins from membrane instead of gel!! Could you tell me glycine which is used in Towbin buffer is compatible with mass spectrometry?