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Questions related from Neda Mohammadi
I did cloning. it was like a traditional approach. Firstly, Restriction digestion was performed for both vector (8kb) and target gen (700bp), and also the vectors and target gene were run on the...
22 March 2020 9,749 4 View
I would like to inhibit the activity of two miRNAs, mir21 and mir133, using Tough decoy method. For this, I have designed some sequences and ordered those oligos, as you can see in attached file....
20 March 2020 9,611 0 View
I did miniprep for a transformed bacteria based on the protocol, like: 1. Transfer 1 ml culture tube ino the epi 2. Centrifuge at 3500 rpm for 10min 3. Add 250 µl Buffer MP1 (resuspend...
30 January 2020 4,925 3 View
In my work, i am working on a serological proteomic analysis (SERPA) project, seeking for the antigens capable of eliciting immune responses. So just like any other typical gel-based proteomic...
28 February 2017 9,480 3 View
In my work, I extracted proteome from breast cancer cell line by lysis buffer and precipitated it with acetomethanol. I use 180 µg of proteins and I have separated it on a 12% sds-page. The...
24 September 2016 4,359 3 View
