PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Please let me know how to remove mycoplasma contamination from cell line?

I am facing a problem in maintaining the cell line of HepG2 and HepG2.2.15. I have used plasmocin Tm treatment (Invivo gen) Cat.#ant-mpt Lot #MPT-41-01 for more than a month. I have also used...

21 February 2021 6,046 4 View

Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

What is the correct way to apply a Design effect for sample size calculation when the sampling method is linear systematic random sampling?

When we are doing sampling by means of simple systematic random sampling,how do we calculate the design effect for use during calculation of sample size for our work?

20 January 2021 5,328 3 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

How to generate .dlg files on PyRx?

Hi. I'm using the PyRx version 0.8 and getting the error message: WARNING: Filename mismatch: "2DFN_ADP_CL.maps.fld" :: "..\2DFN_ADP_CL.maps.fld" C:\Program Files (x86)\The Scripps Research...

17 January 2021 843 3 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Traditional ANN: I obtained high accuracy but using trained model poorly forecasts the unseen data (Binary classification)?

Dear Experts, I trained the neural network with six inputs using the backpropagation algorithm. The target is 0 and 1 which is needed to be classified. The performance of NN model is 98.4% for...

24 February 2020 7,394 7 View

Can any one give me the basic ideas of Genetic Algorithm, Neural Network and Back Propogation Network algorithm?

Hello every one, I want to understand the basic concepts of how the genetic algorithm works and how i can connect genetic algorithm with Neural Network? second I want to...

10 August 2019 9,653 6 View

How to address different constraints using Back propagation?

Dear all, I am trying to use Back propagation for incorporating learning in expert system. However, the expert system has different constraints like bound constraints , inequality constraints. Is...

07 August 2019 7,580 6 View

Can any one give me the basic ideas of Genetic Algorithm, Neural Network and Back Propogation Network algorithm?

Hello every one, I want to understand the basic concepts of how the genetic algorithm works and how i can connect genetic algorithm with Neural Network? second I want to...

07 August 2019 5,135 6 View

How to do classification with backpropagation algorithm in labview?What are the different datas that could be used for this application?

I am looking for a tutorial with detailed explanation or some sample blockdiagrams of labview

07 August 2019 9,483 2 View

How to do classification with backpropagation algorithm in labview?What are the different datas that could be used for this application?

I am looking for a tutorial with detailed explanation or some sample blockdiagrams of labview

07 August 2019 7,614 3 View

What are Vanishing and Exploding Gradients in RNN ?

When using RNN, we often have the problem of vanishing gradient or exploding Gradient. Is there any suggestions or proposition to resolve such as problems? Thank you. kind regards

28 June 2019 9,323 4 View

What are Vanishing and Exploding Gradients in RNN ?

When using RNN, we often have the problem of vanishing gradient or exploding Gradient. Is there any suggestions or proposition to resolve such as problems? Thank you. kind regards

05 June 2019 2,621 5 View

Is backpropagation really used in practical, commercially oriented AI applications?

Hello to all Artificial intelligence is often mentioned as an area where corporations make large investments. It seems that they use AI in autonomous vehicles, face recognition, medicine,...

25 October 2018 1,621 4 View