New project in Cathodic Protection?

What fields (topics) do you suggest to work as a metallurgy and Technical Inspection master degree Student in Cathodic Protection? Thank you kindly

17 January 2020 4,201 5 View

Can we analyse our JPEG data using ImageJ?

Hi everyone.. is it possible to analyse our data saved as JPEG in ImageJ? I did tumourspheres culture under hypoxic condition and have to take the pictures of my spheres in multiple 6 well plates,...

25 March 2018 6,813 2 View

How do I increase protein yield when doing total protein extraction on tumourspheres?

15 February 2016 8,278 3 View

Do I need to change media for cancer stem cell tumoursphere culture everyday?

I am culturing liver cancer cell line in serum free tumour sphere medium (DMEM/F12 + EGF + bFGF) and culturing them for at least 7 days to enrich for cancer stem cells. Do I need to change the...

03 November 2015 4,115 1 View

What are the difference between different HCC cell lines (HepG2, Huh7, PLC/PRF/5)?

I know from ATCC/JCRB depository saying that these cells are different based on their source and the presence of HBV/HCV. What kind of other consideration when I'm looking into working with more...

26 September 2015 2,241 6 View

Can anyone recommend a good quick spheroid formation protocol in 6- well plates?

Hi, I am trying to generate a spheroid to culture HepG2 cells , and my objective is to evaluate the effect of different culture conditions on the marker expression. Not really on the optimisation...

01 August 2015 9,404 17 View

Can anyone recommend a good unconjugated CD133 for multiple applications?

I am planning to do western blot for a start to analyse CD133 expressions in my cells, and therefore are using unconjugated antibody, which I hoped can be used for other applications such as...

05 February 2015 6,365 2 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View