I've came across the fact that egg membranes are extracted by means of dissolving the calciferous shell in acetic acid, but does it truely harm the protein structure of the membrane.
What damage does matter? Structural, physical, chemical? Acetic acid and acetone will likely cause structural damage.
Thank you for your response.
I'm concerned about the structural damaged that may be imparted by the above mentioned solvents, I just needed a medium to wash the membranes without damaging them, and the qualities of the medium should be :
1) It should not damage the membrane
2) It should be volatile
any suggestions ?
The most promising option to me would be a weak acidic aqueous solution and then stripping the membrane from the inside. Good luck.
Yes, the cortex is damaged when high concentrations are used, as these concentrations dissolve the cortex, which leads to a loss of calcium that is supplied to the fetus, in addition to damage to the pores and stomata in the cortex, which contribute to the gas exchange between the fetus and its environment.
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