Is the ChIP assay the only verified assay for chromatin-bound proteins?

10 October 2012 0 10K Report

Currently wanting to look at chromatin-bound vs soluble protein concentrations.

Badges
Science topic

More Katie Brady's questions See All

Does anyone know a method for using DAPI intensity in immunofluorescence to identify cell cycle?

My PI was at a conference this spring where they used the intensity of the DAPI stain of fixed cells to be able to tell the cell cycle phase of individual cells in an asynchronous population. I'm...

06 July 2016 8,517 0 View

What is the method or buffer conditions for lysing mammalian cells for both genomic AND mitochondrial DNA?

Hi all, I am wondering as to the conditions of a lysis buffer that would lyse both the cell membrane and the mitochondrial membrane. The purpose of this lysis is to collect genomic and...

09 October 2014 418 3 View

Bacterial whole cell lysis?

I'm looking for a lysis buffer recipe and protocol for lysing an E. coli strain. The background is that I will be using IPTG to induce the expression of a plasmid with a specific cDNA. I...

10 November 2013 6,167 6 View

Techniques to destain Giemsa-stained cell colonies

I am currently staining cells to quickly look at the effect of a drug for both 24 hour and continuous treatment. At the end of the assay, the cells are to be stained with Giemsa and will be judged...

31 December 2012 2,909 8 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View