Katie Brady

5 Questions 5 Answers 0 Followers

Questions related from Katie Brady

Does anyone know a method for using DAPI intensity in immunofluorescence to identify cell cycle?

My PI was at a conference this spring where they used the intensity of the DAPI stain of fixed cells to be able to tell the cell cycle phase of individual cells in an asynchronous population. I'm...

07 July 2016 8,542 0 View

What is the method or buffer conditions for lysing mammalian cells for both genomic AND mitochondrial DNA?

Hi all, I am wondering as to the conditions of a lysis buffer that would lyse both the cell membrane and the mitochondrial membrane. The purpose of this lysis is to collect genomic and...

10 October 2014 479 3 View

Bacterial whole cell lysis?

I'm looking for a lysis buffer recipe and protocol for lysing an E. coli strain. The background is that I will be using IPTG to induce the expression of a plasmid with a specific cDNA. I...

11 November 2013 6,218 6 View

Techniques to destain Giemsa-stained cell colonies

I am currently staining cells to quickly look at the effect of a drug for both 24 hour and continuous treatment. At the end of the assay, the cells are to be stained with Giemsa and will be judged...

01 January 2013 3,022 8 View

Is the ChIP assay the only verified assay for chromatin-bound proteins?

Currently wanting to look at chromatin-bound vs soluble protein concentrations.

10 October 2012 9,997 0 View