I need to isolate total RNA from PBL of bovine blood for reverse transcription. The blood sample was collected by another lab and shipped to my lab, but it was delayed so that I got the blood one week after the animal was bled.
So, is that possible for me to get good PBL and RNA from this sample?I didn't isolate the PBL yet.
Was it shipped in RNA Guard tubes?...I think you can still do that...So long you are able to retrieve intact cells and able to apply your isolation techniques upon them, you would be able to isolate intact RNA.
@Shahinaz Mahmood Shalaby
Hi Shahinaz, thanks for your suggestion. I did try to isolate PBL with Histopaque, but got nothing with 50ml blood. In fact, we usually isolate cell within 2h after bleeding. But this time we have no choice but to go ahead to see if we are lucky, but no...
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