Based on Promega's manual, I have tried to select colonies of JM109 (Promega Ref. No. P9751 - non competent) on M9-B1 minimal plates for processing to prepare competent cells. I have got the tiny colonies that look like pinpricks. Is that normal and shall I proceed with those colonies or what modifications do you suggest? I have attached the relevant information of protocol and product details.
https://www.promega.in/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109/
Hey,
it´s normal that bacterial growth in M9 medium (or plates) is much slower than in complex medium. Cells should grow well again after inoculation of a LB preculture.
Colonies will take longer to grow, probably 2 days for JM109. But you should get normal size colonies after 2 days. If you don't, then there might be another problem.
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