The TUNEL labeling for apoptosis in the tumor tissues was visualized by BrdUTP incorporation, detected using red fluorescence labeled anti-BrdU monoclonal antibody. However, the overall red intensity is low. I'm wondering whether it's acceptable or not to adjust the overall brightness/contrast for all the groups.
Peter Sobolewski
Linear adjustment of brightness/contrast to the same extent to the entirety of both images (essentially tuning the look-up-table, LUT) is perfectly fine, just don't mess with the gamma, as that can be regarded as a form of manipulation.
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