What are the general antibody concentrations in flow cytometry?

Hi everybody, I am trying to block Fc receptor by human IgG for a specific experiment (it's not flow cytometry), in an adherent cell, not a suspension. I know my primary antibody working...

20 September 2020 9,119 5 View

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

29 December 2019 389 4 View

What is the correct output format of the htseq package?

Hi everyone I used the HTSEQ package to count the reads. The output of this package was as follow: Is this output format correct? Is the first column the gene id and the second column is the reads...

21 December 2019 9,326 4 View

Primer blast from NCBI?

Hello How we can do in Primer blast from NCBI that What size is made of PCR product with D-Loop Primers by using DNA samples from Cervus and Capra aegagrus?

04 October 2019 9,001 3 View

Why did my HUVECs growth rate reduced after transfection?

I did transfection using lipofectamin 2000 to get stable GFP positive cell line, but after transfection and puromycin selection my HUVEC growth rate reduced. I have not seen any colony displayed...

03 June 2019 5,109 5 View

How can I remove the produced HCL during esterification reaction without adding base?

In the esterification reaction that I work on, HCl is produced.Adding base ruins the expected product since it reacts with the initial materials and forms a red byproduct. The bases that I tried...

23 January 2019 4,764 3 View

In cross frequency coupling, is it possible to taking one frequency from LFP and the other from EEG ?

28 June 2018 9,305 1 View

What databases are available for Rna-seq datasets?

I'm looking for any publicly available RNA-seq data sets containing RNA-seq data related normal brast tissues.

31 December 2017 6,029 8 View

Measurement of insulin resistance in diabetic rats?

1- How can insulin resistance be measured in diabetic rats? 2- Why is HOMA-IR not valid for rodents?

19 October 2017 3,092 4 View

Nanomaterials by sputtering with different targets

The paper deals with the manufacture of various nanomaterials by sputtering with different targets

07 August 2017 8,750 3 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View