Is Spirochetes autofluorescent?

26 June 2019 0 8K Report

Does anyone know if bacteria from the Spirochetes genera are autofluorescent--specifically, under ex/em 540/650 (so, red)?

Badges
Science topic

More Katherine E. French's questions See All

Could you recommend some articles on Urban Transportation System optimization and Innovation?

13 August 2024 2,595 3 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Articles on" Gender disparities i leatherwork education"?

Articles on" Gender disparities i leatherwork education"

07 August 2024 2,500 0 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,589 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

Could this be a spirochete?

Fecal grams stain. Better images are available. Keep in mind can range in size - up to 500 um. Thanks!

02 July 2024 9,022 2 View

Eliminate the autofluorescence of red blood cells in mouse spleen paraffin sections?

Immunofluorescence staining of mouse spleen paraffin sections showed strong fluorescence signals in red blood cells in the control section without antibodies. Is it autofluorescence of red blood...

02 July 2024 827 3 View

Hi All, about multiplex immunofluorescence,what could be the reasons for unwated flurophore background ? other than autofluorescence and 1ry Ab conc?

Could the tissue quality/age be an issue ??

15 June 2024 7,763 0 View

How to normalize fluorescence measurements from different microplate runs?

I'm currently doing an experiment where I'm following the expression of a reporter gene (GFP) along growth using S. cerevisiae. The cultivations last around 48 hours. I observed that the results...

10 June 2024 5,941 3 View

How to remove autofluorescence from brain blood vessels in zebrafish?

Hello all, I'm performing brain immunolabeling in 15 days post-fertilization zebrafish larvae by using green and red channels (Alexa Fluor 488 and Alexa Fluor 594) with a 2-photon microscope. I...

28 April 2024 3,569 0 View

Some questions about 7AAD and ANNEXIN-V-FITC to detect cell apoptosis on a flow cytometer？?

I use 7AAD and ANNEXIN-V-FITC to detect cell apoptosis on a flow cytometer. I have some questions I would like to ask you. Regarding the FSC and SSC gates, should the group of cells on the upper...

26 April 2024 4,225 3 View

Can you help identify these layers of the mouse retina?

As a complete newbie to retina research (and imaging in general) - I'd be grateful if anyone could look at this image and offer insight. This slice of a mouse eye shows two fluorescently labeled...

20 February 2024 501 2 View

Why do I see green fluorescence in absence any green fluorophores?

Greetings all! I am seeking help with a question I recently stuck with. In the images below, you can see an example of the immunostaining of brain tissue. There is only DAPI and auto-fluorescence...

04 January 2024 5,160 10 View

Have you ever digest keratin from FFPE tissues without compromising the quality of the tissues and RNA?

Hello everybody! I'm trying to find out if anyone has already digested keratin from FFPE tissue, if so, what would your protocol be? I work in spatial imaging, and I have a problem with the very...

01 January 2024 481 0 View

Does somebody know how to reduce myelin-caused autofluorescence?

We are currently conducting IHC-IF on the mouse spinal cord. However, the autofluorescence caused by myelin is too intense, hindering our analysis of markers. We would greatly appreciate any...

13 November 2023 686 2 View