Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

How to measure protein prescence in a tile scan in ImageJ?

Hello all, I have attached an image in which I would like to know the IntDen and/or the amount of protein particles. The wells are custom made, thus looking slightly warped sometimes. So far my...

10 February 2021 3,478 3 View

What is the red autofluorescence in my HL60 cells?

A subset of my HL60 cells has red autofluorescence in the far red region (FL-3 > 670 nm). I first noticed this some months ago when I was checking an antibody with a PE-Cy5 tag by flow...

06 December 2020 6,005 2 View

How to quantify relative amounts of a substance with immunostainings through confocal microscopy when samples have high autofluorescence?

I am working with plant tissues, buds specifically, fixed in glutaraldehyde and embedded in LR White resin. I am working with the primary antibodies LM19 and LM20 targeting pectins. I am using a...

21 September 2020 5,213 6 View

Do you have any other suggestion to reduce autofluorescence in FFPE tissue?

I used Fluorophore Conjugated primary antibody (Alexa-488, 555 and 647). Tissue FFPE samples were imaged before I stainded antibody and I found background fluorescence. So, my question is "what...

18 August 2020 2,294 3 View

How to deal with autofluorescence of Bouin fixative on paraffin embebbed tissue?

I am working with a mouse prostate and I have autofluorescence problems when using the Bouin fixative. In fact, I'm not sure what the origin of autofluorescence is ... it could be the tissue...

23 July 2020 5,176 1 View

Any advice for reducing autofluorescence in spleen tissue?

Hello, I'm having some trouble with visualizing my IF in spleen tissue. I first found extremely high background and could not differentiate fluorescent cells and background. I then realized my...

23 July 2020 2,187 1 View

How to detect lipofuscin autofluorescence in paraffin embedded tissues?

I am trying to detect lipofuscin on old mice brain. Does anyone know what is the best excitation and emission wavelength to detect it? Thank you.

01 February 2020 1,600 2 View

How do I boost the signal of an Alexa594-conjugated dextran?

I am working on a project tracing projections using an Alexa594-conjugated dextran. I am interested in very fine processes which I'm having difficulty distinguishing from my autofluorescent...

16 December 2019 8,003 3 View

How do I boost the signal of an Alexa594-conjugated dextran?

I am working on a project tracing projections using an Alexa594-conjugated dextran. I am interested in very fine processes which I'm having difficulty distinguishing from my autofluorescent...

11 December 2019 6,379 3 View

Is there any possibility to obserev an specific protein in a autoflourescence microtissue?

Hi every body, I prepared a microtisssue from autoflourescence cells, which when I did control negative flourescence staining for that (without using primary antibody) and using a redemitting...

11 December 2019 4,526 2 View