As a complete newbie to retina research (and imaging in general) - I'd be grateful if anyone could look at this image and offer insight.
This slice of a mouse eye shows two fluorescently labeled proteins (green and red)(x10 objective on slide scanner).
Note the proteins were introduced by viral injection and the red protein should be synaptic.
My intuition is that the red fluorescence here is artefact - due the signal in the choroidal stroma (TOP band) and the diffuse blurry pattern of all red bands. However the signal in what I take to be OPL and IPL is consistent with where this protein might be found.
Perhaps this pattern is autofluorescence of some kind common to these retinal layers? Any thoughts would be appreciated
Thanks
Tommy