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Can we use Artificial intelligence 1. handling or process of samples 2. Microbiology lab for detection of bacteria load 3. technical errors 4. Microscopic examination if yes than how?

10 March 2020 5,560 5 View

Could you please suggest me which will be suitable solvent for good dispersion of TiO2 nanoparticles?

particles size is

02 March 2020 2,566 7 View

What is Q index?

What is Q index of a journal or paper? what are its benefits? explain this with its significance and also pls let me know how to find q index of a given journal

27 April 2019 9,851 22 View

How to isolate Nasal or lung epithelial cells?

Hi All, I need to isolate mouse nasal and/or lung epithelial cells for my experiments. Please share protocols or references regarding this. Thanks in advance, Sudeep

27 March 2019 6,884 3 View

How do I convert .dna files generated from Snapgene software into fasta format? Are there any free softwares for conversion?

I need to use them in fasta format as many softwares consider only this format.

08 October 2018 5,156 3 View

How to increase recombinant protein expression in 293 FT cells?

Hi all, I am trying to increase the production of a recombinant protein in 293 FT cell line. I found some papers that suggest that use of valproic acid, which act as HDAC inhibitor and increases...

09 August 2018 2,025 2 View

How can i calculate the gearbox output torque which is driven by an electric motor?

For more details please find the attached the motor data sheet and the gearbox is worm kind with ratio is 10:1.

16 January 2017 8,075 3 View

Is there any dye which specifically binds bacteria when the bacteria is present along with the mammalian tissue?

I would like to dual stain the infected tissue. So I am searching for the stains that specifically binds to bacteria. Also, the stain should not give the background noise. Thank you.

14 December 2016 2,030 2 View

Why are only the articles published in journals indexed in highly acclaimed databases only given due importance?

Well this question might raise a lot of eyebrows, but still going through lot of free open access journals indexed to lesser known databases i found a lot more innovative and informative articles...

01 January 1970 5,968 21 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How do I plot Pressure against crank angle degree in Fluent?

18 February 2021 8,390 3 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

How to measure acetic anhydride solution with 18M sulfuric acid?

I am doing research on the synthesis of paracetamol. I am preparing acetic anhydride solution with a few drops a sulfuric acid and wish to measure the pH of this solution. However my pH meter...

14 February 2021 8,699 3 View

Best synthesis method for large scale silver nanoparticle synthesis with high yield?.Best post processing steps after nanoparticle synthesis?

I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...

11 February 2021 4,964 13 View

CDNA synthesis or qPCR did not work?

Hi everyone! I extracted RNA from urine pellet using phenol-chloroform method. The yield of RNA was 160ng/uL. 3uL of RNA was used for cDNA synthesis in 20 uL reaction using Go-Script kit....

05 February 2021 6,587 4 View