Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

Why samples do not run in resolving gel?

Hello everybody, I have a problem with SDS-PAGE and I do not know why my samples stop at the top of the resolving gel and they do not run through it. The percentage of gel is 15% and the size of...

14 February 2021 9,116 6 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

Is it possible that the single-pass absorption exceeds 50%?

Dear researchers It's well-known that the maximum light absorption of any structure can not be greater than 50%. Is it possible that the single-pass absorption exceeds 50%? How?

17 January 2021 2,335 7 View

Most appropriate fit of a standard curve in an enzym-linked lectin assay?

Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...

03 January 2021 1,010 6 View

How do I access Domestic Violence Attitude Scales or find them for free?

Hi, I'm a postgraduate student currently starting up a research project on attitudes towards domestic violence (DV). The study will aim to compare cultural differences between attitudes and...

31 December 2020 781 3 View

A good protocol for protein purification by AKTA?

Hi everybody, Does anyone have a good protocol for protein purification using AKTA? I have tried to purify two proteins but after several attempts it was not successful. So, I really appreciate...

28 December 2020 5,220 15 View

How can I calculate group index of one dimensional photonic crystal by using Rsof?

12 July 2020 6,040 2 View

How to measure polarization conversiong efficiency using RSoft?

Hello, I'm trying to measuring PCE by using RSoft. What I wanna measure is Polarization conversion efficiency that is defined as the fraction of the incident circularly polarized optical power...

11 December 2019 4,563 0 View

Can I do meta-analysis of some of the included studies and systematically review the rest?

Hi, For example: If I have 20 eligible studies (Both case-control studies and cross-sectional studies without any control group ) coming out of a systematic search. Can I do meta-analysis with 10...

26 March 2019 6,690 6 View

Can I do meta-analysis of some of the included studies and systematically review the rest?

Hi, For example: If I have 20 eligible studies (Both case-control studies and cross-sectional studies without any control group ) coming out of a systematic search. Can I do meta-analysis with 10...

02 March 2019 4,372 7 View

Bragg grating on optical waveguide?

Which software is better to design a bragg gratings on a waveguide depending on computational time and ease of design? COMSOL or RSoft Beam Prop? and Why? Can anyone suggest?

03 April 2018 1,029 0 View

How can i influence the kerr effect in Rsoft software?

Hello to all, I am trying to simulate a logic gate in Rsoft software.To achieve the best answer,I need to influence the kerr effect on my design,but i dont know where i can influence it?

11 December 2017 1,452 0 View

How to measure intensity of input or output port port using RSOFT?

21 August 2017 6,029 2 View

How to measure intensity of input or output port port using RSOFT?

07 August 2017 4,065 2 View

Is Rsoft software manipulate structures with multi input? if yes how to make more than one source?

Is Rsoft software manipulate structures with multi input? if yes how to make more than one source?

03 April 2017 6,100 0 View

Rsoft Fullwave simulation ?

Hi, I just learn to use Rsoft recently. I did a simulation for Y-spliter rib waveguide structure. My simulation is in 3D. When I launched the fundamental mode with continuous wave excitation type...

06 July 2016 8,407 2 View