Is retinoic acid (RA) or Nerve Growth Factor (NGF) better for Sh-Sy5y cell differentiation?

More Kanna Pichappan's questions See All

Can FBS, kept at 24-26 degree Celsius for about 16 to 18 hours be used for cell culture?

Will that be stable enough ? how can we check for its stability and efficacy?

14 June 2023 8,742 1 View

What is the difference between Total circulatory arrest (TCA) and Deep hypothermic circulatory arrest (DHCA)? which one has more advantages?

In long duration Cardio thoracic and vascular surgeries, Perfusionist and Surgeons induce either TCA or DHCA to prevent ischemic damage and to maintain myocardial protection, among them Which is...

30 November 2022 2,525 5 View

How to model pain in cell culture?

Hi! How do I model pain in cell culture research? I am interested in, both, regular pain and complex regional pain syndrome. Cell lines? Differentiation/activation? Thanks!

02 March 2020 8,514 2 View

Complex Regional Pain Syndrome vs Regular Pain?

Hi! What is the difference between Complex Regional Pain Syndrome and regular pain? I am aware of the various symptoms. However, from a cellular standpoint, what is the difference? Any specific...

02 March 2020 9,682 3 View

Common cell lines for this remyelination and lysolecithin induced demyelination models?

I would like to use a "natural" axon based co culture model to study remyelination and lysolecithin induced demyelination. However, I do not have access to some popular cells lines that are used...

07 August 2019 8,163 2 View

What concentration of betanins do I treat S42 Cells?

I would like to treat S42 cells (schwann cells from the peripheral nervous system that myelinate) with betanins. I have bought the following betanin powder:...

08 May 2019 7,675 1 View

The distance spectrum of the path Pn and The First Distance Eigenvector of Connected Graphs by Subhi N. Ruzieh & David L. Powers, LML 1990?

Can any one share this publication

10 January 2018 2,681 1 View

How we can remove Ammonium Chloride salts from highly water soluble organic compound?

Basically this is organic compound of free base and contains Amide and Amine groups.If we tried Acid base treatment it is not extracting into any organic solvent.

11 January 2016 1,431 8 View

Eastern Indian Mangrove Alliance for Conservation (EIMAC)

Although, mangroves have been acknowledged as effective coastal bio-shields and conservation of the same has been taken up as a collective global agenda[1], a more collaborative and intensive...

01 January 1970 2,219 12 View

Axon based co culture model to study remyelination and lysolecithin induced demyelination In Vitro

I would like to use a "natural" axon based co culture model to study remyelination and lysolecithin induced demyelination. However, I do not have access to some popular cells lines that are used...

01 January 1970 3,154 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good day!! All, can anyone recommend the best battery related journals to download research articles please?

I am an Mphil research student and looking for high impact factor battery related journals to download and read research articles to improve my knowledge and get new ideas in the field of battery...

01 August 2024 6,516 2 View

About impact factor?

I have been publishing the Nepalese Journal of Agricultural Sciences (online ISSN 2091-0428; print ISSN 2091-042X) on www.nepjas.com and research gate. I was wondering how I can get an impact...

01 August 2024 1,277 1 View

Ellen A G Chernoff

You might want to use both: “treatment with growth factors such as nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) has been shown to support differentiation into and maintenance of a mature neuronal phenotype, particularly when used in combination with RA or TPA treatment [2, 23, 24].”. From: Article Considerations for the Use of SH-SY5Y Neuroblastoma Cells in...

Kanna Pichappan

Ellen A G Chernoff Thank you very much!