What should be the percentage of DMSO for dissolving crystal during MTT Assay ?

I am doing MTT assay. I have added 30ul of MTT to cell in 200ul in 96 well plate. After 3hr incubation, I need to dissolve violet color crystal, for that purpose how much percentage of DMSO will...

26 June 2020 2,881 2 View

What is typical range of Fermi energy in Graphene? and Relation between conductivity and Fermi energy of Grpahene?

Conductivity has an inverse relation with Fermi velocity (VF). But Fermi velocity (VF) is directly proportional to Fermi energy.

18 June 2020 8,140 2 View

MATLAB code for Dispersion curve of pipe semi analytcial finite element method??

Can anyone can provide MATLAB code for Dispersion curve of pipe semi analytcial finite element method?

08 December 2019 2,900 3 View

How to mix essential oils with sublimated materials?

I want Mixing an aromatic oil with a sublimation materials that allows the oil to gradually penetrate for the longest period to control insect in lab condition

08 December 2019 6,649 1 View

What is the best transfection method in SHSY5Y cell line?

I want to double transfect two plasmid constructs in SHSY5Y cell line. It is very difficult to perform as I have less than 10% transfection efficiency. Which is the best method to achieve?

14 March 2019 5,732 7 View

How to perform Partitioning of variance to define the role of factors on insect development?

I have performed the experiment where I fed green tomato fruits (natural host) to the Helicoverpa armigera under six different alternating temperatures as treatments viz. 25:10, 25:13, 25:16,...

02 November 2018 4,126 2 View

Which is most preferred for calculating variances and standard error of life table parameters at different treatments? Bootstrap or jacknife?

Recently, I have analysed my results of life table parameters of an insect using jacknife procedure. I have also come across papers analysing same results using bootstrap. I want to know which...

30 August 2018 5,154 3 View

TGE is working but not SGE in DG44 cells ?

Our lab is working on developing the stable gene expressing clone in DG44 cells,. After transfection, transient gene expression is working, after growing the cells in the media with out GHT and...

28 August 2018 9,282 2 View

Is it right to mention transformed values of data, if the data has been analysed using Tukey's HSD post hoc?

I have analysed per cent survival data of an insect and also find out its arc sine transformed values. Is it appropriate to mention these values if I have analysed my data using Tukey's HSD post hoc?

27 July 2018 2,704 3 View

How to get equation for 3D scatter curve in spss?

I have worked upon impact of alternating temperature which is diurnal and nocturnal temperature on insect. I found that I can plot 3D scatter curve but how to get its equation in spss 23 version...

05 March 2018 9,143 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View