Is Qiagen AllPrep DNA/RNA FFPE kit compatible with Laser Capture Micro-dissection using arcturus capsure HS LCM caps?

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How to Dedust Activated Carbon Granules?

Dear all, We are producing activated carbon from hazelnut shells but have an issue about the dust of the a.c. granules also known as the dust film on granules. Linear vibrating seives doesn't do...

11 July 2024 9,782 2 View

PCB manufacturing: How to detect Cu residuals in Ni/Au surface finishing?

Metroology method scanning big areas ~ 10" scale to detect Cu residuals in a metal layer compound with Ni/Auf surface finishing

07 January 2024 938 0 View

How come I get an inverse correlation signal output in my ELISA titration?

Hello, I am identifying nanobodies for my protein of interest. I have identified several different nanobodies showing specificity towards my target and conducted a validation assay (ELISA...

01 December 2023 6,881 1 View

How do I replace a posted file with a revision?

I just deleted an old file and I now see no place on the website to upload my new revision.

07 November 2023 9,862 1 View

What is the best method to measure tourism carrying capacity in national parks or other large natural regions (mountain areas, etc.)?

In your opinion, what is the best method to measure tourism carrying capacity in national parks or other large natural regions (mountain areas, etc.)? What objective criteria can be used to...

19 October 2023 9,457 0 View

What methods can be used to enhance or improve quality control?

Is there any methods that can help quality control enhance its role?

03 September 2023 8,310 1 View

Mini prep plasmid with DNA contamination? How can I avoid DNA contamination trying to QuikChange mutate my plasmid?

Hello, I am currently working on making mutations in my plasmid conducting Quikchange. The plasmid size is 3800bp and I am using primers with several mutations. However, both my parental plasmid...

19 July 2023 5,156 3 View

What are the key differences and benefits of using Seurat and Scanpy for single-cell analysis?

Dear ResearchGate Community, I am currently engaged in single-cell analysis for my research project and would greatly appreciate your insights and experiences regarding the use of Seurat and...

19 June 2023 9,045 5 View

Reactive Oxygen Species in water?

Hello, I just wonder to which extent ROS like OH radicals or H2O2 can be generated in aqueous solutions. I know that there are papers about water radiolysis from 100-200nm, but any ideas about...

02 March 2023 7,927 2 View

What is the negative controls in phage display (biopanning) and troubleshooting panning protocol?

Hi there, I am currently panning against GFP and pre-depleting with PBS+0.5%BSA using the steps attached (modified from Barbas's protocol). GFP and BSA are immobilised on high-binding microtiter...

24 January 2023 388 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View