Looking for high efficient light sources (Laser, HE LED) for Spectroscopic Ellipsometry?

Conventional light sources have a penetration depth of a few nanometers in metal layers and cannot be used for spectroscopic ellipsometry purpose to measure layer thicknesses of a few 100 nanometres.

27 October 2020 7,663 4 View

Why is fungal/bacterial DNA isolated with Qiagen Powerlyzer Powersoil Kit not stable?

We have isolated DNA with the Qiagen Powerlyzer Powersoil Kit (with addition of Proteinase K) for NGS downstream applications (16SrRNA/ITS2) and encountered the problem that the isolated DNA is...

26 October 2020 7,418 2 View

Comparing the constructs by using different scales of measurement in different samples?

I was wondering whether it would be possible to measure the same construct (i.e. the perception of training) in two samples by using different scales for each sample (i.e. employed and unemployed...

02 May 2020 3,917 10 View

GHRH/GHRF antibody for rat brain immunohistochemistry?

I'm looking for an antibody (preferably validated) for growth hormone releasing hormone (GHRH/GHRF) for immunohistochemistry in rat brains. Do you have any leads? I would really appreciate your...

18 June 2018 5,384 1 View

How to culture cells before alizarin red staining.?

08 May 2017 5,630 7 View

How can I stain for dead neurons in the ischemic mouse brain?

I am looking for a staining method to label dead neurons in the ischemic mouse brain.I use coronar cryosection from mice which were subjected to transient MCAo and reperfusion. I tried NeuN+TUNEL...

09 June 2016 7,426 3 View

Is it possible to use desert sand as a building material?

Like mortar or concrete? I can not find case studies or articles about this topic?

15 May 2016 8,157 27 View

Are there any (high quality) potential energy surfaces for singlet electronic excited states of hydrogen sulfide (H2S) available?

22 February 2016 3,428 3 View

Is there recent knowledge about social structure within a litter of warthog piglets?

Young warthogs (Phacochoerus aethiopicus) display suspicious light colored bristels at their cheeks. In our view these seem to mimic the prominent tuskers of the adult...(?) I would greatly...

06 October 2015 1,983 4 View

Has anyone ever tried to detect Blue Jets, Elves and Sprites above Volcanic Eruption Clouds ?

Many years back, I attended a special session on Elves, Sprites and Blue Jets at AGU in San Francisco. These are typically observed above severe thunderstorm clouds and are observed at something...

27 August 2015 4,229 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View