Hi, I was wondering if there was a sensitivity difference toward the toxicity of DNA damaging drugs between nude mice and NRG mice (rag1 ko). I already know that NRG are less sensitive than NSG, due to the SCID profile of those mice who are, for example, suffering from high toxicity after radiation therapy. So basically, i would like to know if someone knows if the nude mice (Foxn1 ko) are less sensitive to DNA damaging drug side effects than NRG mice (regardless the engraftment ability of these two strains for xenograft)? Thank you
Hi Romain Pacaud - Sensitivity towards the toxicity of DNA damaging drugs is very high in NRG mice (rag1 ko) comparing to Nude mice.
Please find the detail information for your kind perusal
nude” mice are homozygous for the Foxn1nu, or “nude,” mutation. Foxn1 encodes a transcription factor required for both hair follicle and thymic development. In its absence, mice are both hairless and athymic. Because the thymus fails to form, there is no place for CD4+ and CD8+ T cells to differentiate and mature, making nude homozygotes T cell-deficient.
“Scid” mice are homozygous for the Prkdc scid mutation. The gene Prkdc encodes the catalytic subunit of DNA-dependent protein kinase that is required for DNA repair and for sealing the double-stranded DNA breaks that occur during somatic recombination of T cell receptor (TCR) and immunoglobulin (Ig) genes. In the absence of Prkdc protein, TCR and Ig genes cannot rearrange, resulting in mice that are both T and B cell deficient.
“Rag-deficient” mice are mice that fail to express functional Rag1 or Rag2 proteins. Like the Prkdc gene, both Rag1 and Rag2 are required for somatic recombination of TCR and Ig genes, and the absence of either gene results in T and B cell deficiency. Mice that carry either the Rag1tm1Mom or Rag2tm1.1Cgn mutations have very similar, if not identical, phenotypes .
Thank you Somesh for your answer. Actually, like I said in my question, I already knew that NSG mice are really sensitive to DNA damage therapies, but I read that NRG don't have this problem, and the info that you brought don't help enough to be sure about it. Is the fact that Rag1 is involved in TCR recombination has a link with DNA repair?