Dear Sajid,

Dealing with salt treatments in the lab may be a tricky business.

The intensity of applied salt stress (in terms of NaCl mixture) must be chosen in relation to the objectives of your experiment, selected species/cultivar, the moment when starting to apply this kind of treatment. From the data you indicated, the value of EC seems slightly at the conventional cborder between non-saline and saline; however, this would rather apply to ecophysiological conditions, than to  lab conditions. 

Nevertheless, I suggest to check carefully the literature dealing with salt stress - you will certainly find many combinations of experimental designs. You will certainly find data about rice under salt stress conditions.  I suggest to deal mainly with concentrations of NaCl expressed as mM, as you then easily compare your results with others, in a standardized manner. Then, you have to decide if you are going to apply the salt stress starting directly from the seedling stage - usually, the seedlings are more sensitive than adult stages; in this case, perhaps, among others, you should apply the salt treatments later, after seedling emergence and development. Salt stress is very critical for seedlings, even for those of halophyte species.

Thus, there are many factors you should consider in order to achieve a better picture about how to organize an appropriate experimental design related to salt stress. 

Hope that something here mentioned would help you.

Best,

Marius

@Marius Nicusor Grigore THANKS SIR. i developed salt stress in pots at the rate of 10.89mM,  32 mMI and 46 mM. Salt applied before transplanting in pots. may be varitries or sensitive to salt stress

Hi i used water with 20 ds/m NaCl in 4 alfalfa cultivars sevral times in months but they were alive more than 1 months after that i have to  take a sample, then cut all of them.

Potential (bare) = - 0.36* Ec salts solution of -5 bars is the breaking point in biomass in some crops ad in some other slightly less and/or higher it depends om your crops susceptibility to salt for instance -5 for tomato  too much less for cucumber 

 I think something went wrong in the salinity imposition to soil or your rice variety is highly sensitive to salt stress. You can check which one of the above is true for you. I used another method, although in groundnut. You may try it, I provided the link.

ebscohost.com/.../low-ca2-na-rati...

THANKS to all . i think my rice cultivars are more sensitive to salt stress.  their growth is just effected at 4.64 dS/m soil is clay loam with original EC 0.089. i think it is due to high sensitivity of rice at vegetative stage.

Thank you sajid

I agree with Marius with respect to very first statement . Evaluation of genotypes for salt stress in pots , very often fail to stand under filed conditions , unless some some biochemical or physiological parameters are taken into account . Simple morphometric evaluation often produce confusing  results , for which you should be very careful about . In filed , movement of salts has different dynamics than pots..?

EC 4.64 ds/m is not sever salt stress to cause what you had in your experiments. To have salt stress, either in water or soil, it starts from 4 ds/m = 40 mM, and therefore 4.64 ds/m is low salt stress and actually rice is salt sensitive crop. I think something went wrong in your experiment, and I guess it is salt is concentrating in the soil because of water evaporation and you did not leach your soil with water frequently. Actually, what you have in your soil is not 4.64 but more and more due water evaporation. Did you leach the salt from time to time? I think it is not a matter of your cultivar is so sensitive because even sensitive crops can tolerate  to some extent such salt stress you used (4.64) and not die. This may happen after a long time of salt exposure with the correct  salt level 4.64.

I hope I add something of help for you.

Greetings

@M. M. F. Mansour  thank you so much. my experiment is pot cultured. so leaching is not possible and i am irrigating after every two days. so on this salt concentration or EC some plants are still alive. i think evaporation is also a factor and cultivar sensivity

I agree with M. M. F. Mansour. You can leach soil by washing with water every three days to prevent salt accumulation

@Omaima Sayed Hussein · but madam it is not possible to leach soil by washing due to pots. but i am just giving heavy irrigation

Applying 15% extra of required water is highly recommended for leaching salts out of rhizophere 

Ok, heavy irrigation wash the excess of salt through aeration pore in pots