I am looking for Microscopes/Optical Detection which allow the detection of Molecular Beacons on a Microfluidic platform?

I am planning on viewing several molecular beacons on a microfluidics systems and l was wondering if it is important to by an actual microscope or if systems exist that can be used for fluorimetry?

06 July 2015 7,867 11 View

Looking for Mycobacteria tuberculosis specific sequences which can be used for isolation of MTB DNA from a sputum sample?

09 October 2014 6,507 6 View

What does a positive none template control in PCR reaction mean?

I am doing PCR using the Hot start KOD polymerase from Merck and l am getting a positive reaction in the NTC repeated from cycle 36-38 in a 45cycle PCR. I am working in a unidirectional manner...

05 June 2014 9,579 8 View

Does anyone have an idea where l could get reliable DNA from drug susceptible and resistant isolates of Mycobacterium tuberculosis?

Need to perform PCR on these samples.

01 February 2014 1,676 3 View

Is there any method that can be used to isolate DNA without the use of the centrifuge?

DNA prep to be used for PCR.

31 December 2013 2,728 11 View

What polymeric material other than Agar can be used in the construction of salt bridges in Microbial Fuel Cells, preferably cheap options.

I am moving away from Agar because it does not efficiently prevent migration of mediator Methylene between the anode and cathode compartments, thus my quest to use a better alternative. l have...

06 July 2013 9,448 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

No title

02 March 2021 3,060 3 View