I am moving away from Agar because it does not efficiently prevent migration of mediator Methylene between the anode and cathode compartments, thus my quest to use a better alternative. l have been using 10% Agar with 5% Sodium Chloride (all w/v %) in the salt Brige.
Have you considered using a cationic exchange membrane instead of a salt bridge. I once constructed a microbial fuell cell using methylene blue as an mediator, where there were two flat compartments separated by a cationic exchange membrane and carbon fiber was used as electrodes in each chamber. I attached a diagram of the one we constructed.
If CEM is not available in your lab, you may try Gore-Tex cloth, which can be found in many clothes for outdoor sports. It works for MFC, but I am not sure of its function for methylene blue. For reference, http://www.sciencedirect.com/science/article/pii/S0960852409018033
Thank you very much Michael for your reply, it might take me a very long time to order and receive the cationic membranes thus my attempts to use polymeric material. The diagram you attached is going to be very helpful thank you sir.
Thank you Jan and Zheng l am studying your references for any ideas. As you guys have said migration is concentration dependent, so l will try to reduce the concentration in the flasks to 0.08mM.
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