7 Questions 16 Answers 0 Followers
Questions related from Ian Mbano
I am planning on viewing several molecular beacons on a microfluidics systems and l was wondering if it is important to by an actual microscope or if systems exist that can be used for fluorimetry?
07 July 2015 7,955 11 View
I am looking for a sequence (s) l can use for extraction of MTB DNA from a sputum sample using either the Magvigen-Streptavdin-biotin DNA capture kit, or the MagVigen-Plasma DNA Select Kit. To my...
10 October 2014 6,563 6 View
I am doing PCR using the Hot start KOD polymerase from Merck and l am getting a positive reaction in the NTC repeated from cycle 36-38 in a 45cycle PCR. I am working in a unidirectional manner...
06 June 2014 9,686 8 View
I am trying to minimise photo bleaching of the real-time PCR signal but l not sure of the effect of this inhibitor on the general PCR reaction. I am trying to mimic the effect shown in Figure 5 IN...
03 March 2014 4,609 0 View
Need to perform PCR on these samples.
02 February 2014 1,734 3 View
DNA prep to be used for PCR.
01 January 2014 2,808 11 View
I am moving away from Agar because it does not efficiently prevent migration of mediator Methylene between the anode and cathode compartments, thus my quest to use a better alternative. l have...
07 July 2013 9,510 3 View