Is LB medium considered specific media for Ecoli isolation from feaces?

More Nouran Atef's questions See All

statisticaly significant t test results but no fold change?

For example, measuring lymphocytes conc. in control gp was 100 ug/ ml while group 1 average was 115 ug/m, performing student t test results were signficantly different. But how to explain this in...

05 June 2018 3,432 7 View

How to perform Dunnett test on minitab?

I have several groups of treatments, I want to know if each of them is significantly different than the one control group or not, I was advised to use dunnett's test, I wish to know the steps on...

04 May 2017 8,744 4 View

Does the presence of "levan regulatory protein" necessarily means the presence of levansucrase gene in the same organism?

In the NCBI, I'm looking for the coding sequence of levansucrase enzyme in a certain organism ( ex: enterobacter sp.) ..but instead I usually find the coding sequence of "levan regulatory protein,...

03 April 2015 9,862 1 View

What is the quickest way to gather protein sequence data from NCBI in fasta format?

I am using NCBI to search for protein sequence of certain enzyme. The results were over 1000. How can I get all their fasta format in few quick steps?

09 October 2013 10,004 10 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Michael J. Benedik

LB is not selective media for E. coli, because many different bacteria will grow in LB. Often MacConkey agar or EMB are used as selective media for enteric bacteria.

Juan Guillermo Cubillos-Hinojosa

EMB usually are used as selective media for enteric bacteria, and other like Maconkey, E.C., etc.

Brijesh Mishra

Dear Nouran,

LB media is used for growth of auxotrophic mutant. For selective isolation of E.Coli use some selective media like EMB, MacConkey as Anthia,juan said.