Is it safe to use tumors stored in RNAlater for lysate preparation/protein extraction ? Will it affect the quality/yield of protein ?

22 December 2020 1 745 Report

I stored tumors in RNAlater. However, I want to use these tumors for making protein lysates for cytokine expression analysis.

Shobha Devidas Surbhaiyya

Hello dear, Tanvi Visal. First thing If u stored your sample in proper way i.e sample stored in deep freeze or using liquid nitrogen and second thing if u use proper protocol for extraction of protein I think your protein quality and quantity will be good ......good luck

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