Otávio Aguiar Souza

4 Questions 1 Answers 0 Followers

Questions related from Otávio Aguiar Souza

Why my yeast strain is not losing the URA3 gene in 5-FOA plates?

Hello all. I am repeatedly streaking one yeast strain (MATa MAL2-8C, SUC2) in 5-FOA plates aiming to disrupt the URA3 gene. After spreading thrice, the yeast is still able to grow in SD-URA...

05 February 2025 6,045 2 View

Is it safe to make a glycerol stock from Agrobacterium transformed cells from a 10-day plate?

Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...

27 May 2024 9,343 3 View

Is it safe to purify plasmids of E.coli precultures from 2-month old plates?

Hello all. I purified some plasmids after doing precultures of strains from a 2-month old plate in -4 degrees. The PCR results were fine and I used theses plasmids to transform Agrobacterium...

27 April 2024 8,475 3 View

False positive in yeast transformation?

Recently I performed an yeast transformation and I got a few colonies in the selective plate. The strain is very modified, with four auxotrophic markers (CRISPR) and we are struggling to insert...

24 August 2023 2,719 2 View