Otávio Aguiar Souza

3 Questions 1 Answers 0 Followers

Questions related from Otávio Aguiar Souza

Is it safe to make a glycerol stock from Agrobacterium transformed cells from a 10-day plate?

Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...

27 May 2024 9,331 3 View

Is it safe to purify plasmids of E.coli precultures from 2-month old plates?

Hello all. I purified some plasmids after doing precultures of strains from a 2-month old plate in -4 degrees. The PCR results were fine and I used theses plasmids to transform Agrobacterium...

27 April 2024 8,469 3 View

False positive in yeast transformation?

Recently I performed an yeast transformation and I got a few colonies in the selective plate. The strain is very modified, with four auxotrophic markers (CRISPR) and we are struggling to insert...

24 August 2023 2,716 2 View