Is it right to have a value higher than 100% for beta cell function calculation by HOMA software?

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Is there any good volume of data of soil heavy metals/trace metals with GPS points available online as open access?

Hi ! Can anyone provide me any insight about availability of published/open access data of soil heavy metal/ trace element concentration with the GPS location? We are developing a model and need a...

19 December 2018 4,653 5 View

Hello everyone, Is it mandatory to have same mutant sequence in F1 generation fish to cross between them? how can I seperate fish using sequence data?

I have got mutant fishes with CRISPR-cas9 system, and my fishes are now in F1 generation. I have sequenced the mutant region. But I am facing problem to find out the number of base deleted or...

27 September 2018 6,993 1 View

Hello everyone, I have a question regarding gRNA design. Is it okay to select gRNA from last exon??

I would like to design gRNA for gene knock out in Zebrafish. Please guide me about gRNA selection. I am using CRISPRscan for gRNA selection.

01 March 2018 3,661 3 View

In absence of any ancillary data, what information can be extracted from satellite images (LISS III/ IV) for soil pollution study around a landfill?

Some geochemical data are available in a given area around a landfill site. geology, geomorphology maps/information are not available. In such a situation how can remote sensing or satellite image...

21 May 2014 3,911 20 View

How to digest fish muscle samples quickly, easily and in a cost effective way for heavy metal analysis using AAS?

I do not have much laboratory set up. Hence kindly let me know how to prepare a sample using minimal chemical and least sophisticated instruments.

21 February 2014 6,093 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View