Is it recommendable to use NHS/EDC chemistry to conjugate CGKRK to nanoparticle surface ?

11 December 2019 1 9K Report

I am working on PLA-PEG nanoparticles and wondering if I can do NHS/EDC chemistry to conjugate CGKRK to acid terminated end of PLA-PEG. Majority of the reports are on using the thiol group in Cysteine to work out a thiol-maleimide click reaction. I am wondering if that is due to the fact that NHS/EDC might perturb the free amine residues of the lysine thereby rendering the peptide unfunctional.

Omar Gonzalez-Ortega

Indeed lysine residues will be used for the coupling. Coupling using maleimide chemistry will guarantee full peptide exposure.

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