Is it possible to use EDTA tube for collecting whole blood for mRNA extraction? How comparable is it with Tempus tube?

More Wan Shahriman Yushdie Wan Yusoff's questions See All

How teachers can contribute to curriculum design and the benefits of their involvement to student learning and the quality of instruction?

Write a reflective essay on the importance of teacher involvement in curriculum development

15 June 2024 4,240 7 View

What are the black stuff in cell culture?

Hello. I am new to cell culture work. May I know whether these small black dots are contaminants or just cell debris? It's quite confusing because they are not moving. This is different from the...

02 May 2024 8,479 1 View

Why does the EPR spectrum of MnO2 powder exhibit such wide peaks?

As known, the spin exchange coupled Mn4+ leads to a narrowd singal, at ca. g=2.006 with the line width about 500 gauss. Why the birnessite MnO2 dispalys such wide spectrum? Is any other exchange...

23 April 2024 6,351 3 View

What is the standard electrode potential of the silver wire reference electrode?

The silver wire has been used as a reference in an organic electrolyte in many artcles. What is the standard electrode potential of the silver wire reference electrode and how to calibrate its...

14 April 2024 8,052 3 View

Autoclave reactor for mineral carbonation purpose?

Hello, can someone help me to get this autoclave reactor service for mineral carbonation purpose (in Malaysia or outside Malaysia)? For your information, I am using a solid plug sample for this...

12 October 2023 4,405 0 View

Appropriate type/approach for qualitative study?

I am planning to conduct a qualitative study that basically aims to develop the Sustainable Development Goals (SDGs) Literacy Framework for the Agriculture Curriculum. The framework will explain...

10 September 2023 6,399 3 View

How to generate cancer cell line with different HLA-A subtype?

Dear Experts, I would like to know is it possible to generate a cancer cell line (which is HLA-A:02 negative) to be HLA-A:02 positive by stably overexpressing HLA-A:02 gene into the cell line,...

21 August 2023 3,580 0 View

Questions about hypertrophic stimulators of neonatal mouse cardiomyocytes?

To analysis the response and mechanisms of cardiomyocyte hypertrophy, our team tried to induce hypertrophy by adding adrenergic agonists to the medium of neonatal mouse cardiomyocytes. And...

06 June 2023 3,725 1 View

How to calculate DSC lattice vectors in the GB plane?

Previously, I used Frank formula D=b/2sinA to determine. For example, D=a*(z1*z1+z2*z2+z3*z3)/sqrt(z1*z1+z2*z2+z3*z3), where z1-z3 is the crystal orientation along GB normal z. This is successful...

06 April 2023 5,848 1 View

Battery Charger-Constant current?

Hello, My name is Wan. My focus research area is on Battery chargers. I would like to know the part for Constant Current charging. As i have done a simple circuit for the cut-off battery charger...

29 March 2023 4,562 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

The Curse of Evolution and Complexity?

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For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

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The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

How to start a Molecular Dynamics Simulation?

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How to draw the five chelate rings of [Co(EDTA)]- on this picture?

Is this circle one of the rings?

03 August 2024 5,988 0 View

Craig M Neville

Even a microfuge tube would be fine for long term storage at -80 C. EDTA is neither beneficial nor detrimental - it prevents clotting (but your cells lysed when you froze them) and inhibits DNAses (but not RNAses). Tempus tubes are designed for temporary RT storage of blood samples for when immediate freezing is impractical (presumably not an issue here). Tempus also reportedly enriches the RNA from gDNA and protein, but your RNA isolation protocol does that too.RNA isolation isn't rocket science anymore!

Wan Shahriman Yushdie Wan Yusoff

Hi, thanks for answering!

Samples were collected into EDTA tube which immediately aliquots into cryovials prior to storage at -80 C.

Alexander Sasha Vlassov

please email my colleague Tim: [email protected] - he knows everything about Tempus tubes and blood samples