Can anyone suggest me that why protein is not active after purification?

I am purifying a protein which is showing activity in sonicated supernatant but when i am purifying it using ion exchange, eluted fraction is losing the activity.I did dialysis also even then the...

11 December 2016 2,508 5 View

How to visualize the bands of protein on SDS gel other than by coomassie staning?

07 August 2016 8,541 6 View

What can be the reason of not getting induction in expression hosts ??

01 February 2016 7,283 6 View

How to preserve thermophilic organisms?

08 September 2014 2,580 6 View

Why is the DNSA method showing no activity of my isolate although it shows activity on a starch azure plate?

01 February 2014 381 5 View

Heavy metals detoxifying enzymes assay.

I want to check the activity of enzymes responsible for heavy metals detoxification can anyone tell me the assay which i should use for the same.

03 April 2013 2,498 11 View

How to separate ligated fosmid vector from genomic DNA of host cells

I am trying to isolate Fosmid DNA from my clones by using a Fosmid purification kit but every time I am getting two bands on the gel so I am not able to give my samples for sequencing. How can I...

11 December 2012 249 23 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View